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KMID : 0369820100400060333
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Jorunal of Korean Pharmaceutical Sciences
2010 Volume.40 No. 6 p.333 ~ p.337
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Preparation and Characterization of Genetically Engineered Mesenchymal Stem Cell Aggregates for Regenerative Medicine
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Kim Sun-Hwa
Moon Hyung-Ho
Chung Bong-Genn
Choi Dong-Hoon
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Abstract
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Combining cell- and gene-based therapy is a promising therapeutic strategy in regenerative medicine. The aim of this study was to develop genetically modified mesenchymal stem cell (MSC) aggregates using a poly(ethylene glycol)
(PEG) hydrogel micro-well array technique. Stable PEG hydrogel micro-well arrays with diameters of 200 to 500 μm
were fabricated and used to generate genetically engineered MSC aggregates. Rat bone marrow-derived MSCs were transfected with a green fluorescent protein (GFP) plasmid as a reporter gene, and aggregated by culturing in the PEG hydrogel micro-well arrays. The resultant cell aggregates had a mean diameter of less than 200 μm, and maintained the mesenchymal phenotype even after genetic modification and cell aggregation. Transplantation of MSC aggregates that are genetically modified to express therapeutic or cell-survival genes may be a potential therapeutic approach for regenerative medicine.
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KEYWORD
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Cell and gene therapy, Mesenchymal stem cells, Micro-well array, Cell aggregates
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